Her condition started deteriorating Tuesday and she died Wednesday afternoon. We got the confirmation that the woman was suffering from swine flu only Wednesday night,' added Prasad.

According to hospital sources, the National Institute of Mental Health and Neuro Sciences (NIMHANS), where the test was done, confirmed that Rupa was suffering from H1N1 virus late Wednesday night.

Rupa worked as a teacher in the Sudarshan Vidya Mandir at Jayanagar here.

According to figures available with the health department, 522 tests have been conducted in Karnataka and 97 people have tested positive for the H1N1 virus.

With this, India's swine flu death has reached 20. Apart from 15 deaths in Maharashtra, one death each has been reported from Bangalore, Ahmedabad, Vadodara, Chennai and Thiruvananthapuram.

A 75-year-old woman died Thursday in Pune's Sassoon Hospital though it was not immediately clear if she was suffering from the viral disease.

Bharti Goyal died just before noon. She was on the ventilator in the intensive care unit (ICU), Joint Director (Health Services) P.S. Chavan told IANS.

Goyal had been shifted to Sassoon Hospital from the KEM Hospital Wednesday with breathlessness.

Maharashtra Chief Minister Ashok Chavan said Bharti Goyal's swine flu test report had not been received yet.

Since Reeda Sheikh, 14, died of swine flu in Pune Aug 3, authorities across the country have been on alert and efforts are on to contain the spread of the virus.