Back-up sample for future testing should be kept at -700 C.
Sample transport: Absorbent cotton, tissue paper or waste newspaper for wrapping primary container. Secondary container to hold the primary container like bigger tube or sealed plastic bag. Insulated ice box with ice pack, sample proforma fastened on to the secondary container.
Sample handling and testing guidelines:
Handling: In BSL-3 bio-safety or BSL-2+ facility with BSL-3 precautions.
Testing: Real time RT-PCR (Reverse Transcription, Polymerase Chain Reaction) test, by Real Time PCR machine using validated reagents accessories and protocol as per the Centre for Disease Control (US) or World Health Organisation guidelines.