The swine flue claimed the first victim in the state when Pravin Patel, a non-resident Indian (NRI) from Atlanta, the US, died in the Ahmedabad civil hospital early Sunday.
A seven-year-old girl named Arya died Tuesday in Vadodara, about 150 km from here.
In Vadodara, the Delhi Public School (DPS) was the first school to announce closure as a preventive measure.
There are a total of 14 swine flu positive cases in Gujarat. The latest among them is a 22-year-old man from Vejalpur locality of Ahmedabad who contracted the infection from his Pune-based relatives.